ABSTRACTMicrofungi were isolated from unpolluted and copper polluted forest soils and their tolerance to Cu was tested on agar plates. Species from polluted sites were usually tolerant to Cu. The most tolerant species werePaecilomyces farinosus, Beauveria bassiana, Verticilliumspp. andPenicillium brevicompactum.Sensitive species were found amongMortierella, OidiodendronandPenicillium.They were usually rare in polluted sites. Little evidence for adaptation to Cu was found among isolates from sites with short or long history of pollution.