Stability of rat lung and liver microsomal cytochrome P‐450 enzyme activities to storage: Purified microsomal fraction, postmitochondrial fraction, and whole tissue
作者:
Jean Danner‐Rabovsky,
RobertD. Groseclose,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1982)
卷期:
Volume 10,
issue 4-5
页码: 601-611
ISSN:0098-4108
年代: 1982
DOI:10.1080/15287398209530279
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The ability to maintain cytochrome P‐450 enzyme levels in stored tissue samples is necessary in order to properly utilize this enzyme system in toxicological and environmental health studies. The stability of enzyme activities associated with microsomal cytochrome P‐450 was investigated to determine the best way to handle large numbers of samples and postmortem or biopsy lung and liver tissue. Long‐term storage in cold buffer resulted in diminished activities in purified microsomes and whole tissue. Benzo[a]pyrene hydroxylase and 7‐ethoxycoumarin deethylase, however, were stable when the purified microsomal fraction, postmitochondrial fraction, or whole tissue was stored at —80°C, but care should be exercised in the freezing and thawing procedures.
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