Preliminary results obtained with a No-Label, Homogeneous, Optical Immunoassay for Human Immunoglobulin G
作者:
Ranald Sutherland,
Claus Dähne,
John Place,
期刊:
Analytical Letters
(Taylor Available online 1984)
卷期:
Volume 17,
issue 1
页码: 43-53
ISSN:0003-2719
年代: 1984
DOI:10.1080/00032718408065263
出版商: Taylor & Francis Group
关键词: Optical Immunoassay;Total Internal Reflection;Evanescent Wave Immunoassay.
数据来源: Taylor
摘要:
A novel optical immunoassay system was developed and tested using human IgG as a model antigen. Following adsorption of antiserum to the surface of an optical waveguide, the immobilised antibody was then reacted with a solution containing antigen. The reaction was detected utilising the evanescent wave component of a light beam totally internally reflected within the waveguide. The growing antigeh-antibody layer resulted in an increase of scattered light which was monitored kinetically. Individual measurements were completed within 5 minutes and the resultant dose response curve had a sensitivity limit of approximately 10 nmol/L.
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