Enantiomeric resolution of four racemic local anesthetics bupivacaine, mepivacaine, prilocaine and etidocaine were investigated on cellulose based chiral stationary phases. The phases studied were cellulose tris-3,5-dimethyl phenylcarbamate (Chiralcel OD) and cellulose trisbenzoate (Chiralcel OJ). Baseline separation (Rs>1.5) were achieved for bupivacaine and prilocaine enantiomers on Chiralcel OD using hexane-ethyl alcohol (99:1, v/v) and for etidocaine enantiomers on Chiralcel OJ using hexane-methanol (98:2, v/v). Etidocaine and mepivacaine enantiomers only gave partial separations (Rs≤0.81) on Chiralcel OD. The effects of various protic and aprotic organic modifiers were investigated on resolution and retention of the enantiomers.