Fertilization of cumulus‐free, zona‐intact mouse ova in vitro at high and low sperm concentrations
作者:
J. D. Stanger,
P. Quinn,
期刊:
Gamete Research
(WILEY Available online 1982)
卷期:
Volume 5,
issue 1
页码: 61-70
ISSN:0148-7280
年代: 1982
DOI:10.1002/mrd.1120050107
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: mouse;spermatozoa;concentration;fertilization;ova
数据来源: WILEY
摘要:
AbstractThe effect of varying the sperm concentration between 2 × 105sperm/ml and 8 × 106sperm/ml on fertilization of cumulus‐free, zona‐intact F1 (CBA × C57BL) mouse ova by QS and F1 (CBA × C57BL) mouse spermatozoa was studied. The spermatozoa from both strains of mice exhibited optimal fertilization rates at 2 × 106 sperm/ml. However, at sperm concentrations greater than 4 × 106 sperm/ml and less than 1 × 106 sperm/ml, fertilization rates were significantly reduced. F1 spermatozoa were more susceptible to dilution than QS spermatozoa. A significant interaction between strain and sperm concentration indicated that the two strains produced different fertilization rates at different sperm densities.Extracts of epididymal fluid, medium from capacitated spermatozoa, or ampulla fluid did not improve the fertilization rate at 2 × 105 sperm/ml, but retaining the cumulus oophorus did. The decrease in fertilization rate at 8 × 106 sperm/ml can in part be attributed to a nondialysable inhibitor from the neat sperm preparation that appeared to be of epidi
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