Passive Transfer of Adjuvant-Induced Arthritis Into Irradiated Da Recipient Rats
作者:
CannonGrant W.,
HarperD. Scott,
ClaytonFrederic,
GriffithsMarie M.,
期刊:
Autoimmunity
(Taylor Available online 1993)
卷期:
Volume 15,
issue 4
页码: 267-274
ISSN:0891-6934
年代: 1993
DOI:10.3109/08916939309115748
出版商: Taylor&Francis
关键词: Animal models;Adjuvant-induced arthritis;Irradiation;Passive transfer;DA rats
数据来源: Taylor
摘要:
Adjuvant-induced arthritis (AIA) can be passively transferred in Dark Agouti (DA) rats by spleen and lymph node cells after culture with Concanavalin A (Con A). A model not requiringin vitroCon A expansion and activation would be important in investigations of anti-rheumatic drugs in AIA. A new model using irradiated recipients fills this need. Donor DA rats treated with 0.1 ml complete Freund's adjuvant (CFA) containing 7.5 mgM. butyricum/ml were sacrificed 11 days after CFA injection, donor spleen cells harvested, and donor spleen cells injected intravenously into recipient DA rats previously irradiated with 5 Gy. Recipient rats developed arthritis 4-14 days after spleen cell transfer. This model can now be used to further define the effects of anti-rheumatic drugs in the passive transfer of AIA by eliminating the need for thein vitroCon A-induced expansion and/or activation of donor cells.
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