Tyrosine hydroxylase antisense gene therapy causes hypotensive effects in the spontaneously hypertensive rats
作者:
Toshio Kumai,
Tomonori Tateishi,
Masami Tanaka,
Minoru Watanabe,
Hiromi Shimizu,
Shinichi Kobayashi,
期刊:
Journal of Hypertension
(OVID Available online 2001)
卷期:
Volume 19,
issue 10
页码: 1769-1773
ISSN:0263-6352
年代: 2001
出版商: OVID
关键词: antisense;catecholamine;hypertension;spontaneously hypertensive rats/inbreds;tyrosine hydroxylase
数据来源: OVID
摘要:
We investigated the effect of antisense oligodeoxynucleotides (AS ODN) against tyrosine hydroxylase (TH) on hypertension and sympathetic nervous system activity in spontaneously hypertensive rats (SHR). Systolic blood pressure (SBP) in SHR treated with TH AS ODN (50, 200 μg/rat, i.v.) was significantly lower than that of control SHR. Epinephrine and norepinephrine levels, TH activity, and TH protein levels in the adrenal medulla of SHR were reduced concomitant with TH AS ODN treatment-induced changes in SBP. In contrast, TH AS ODN (200 μg/rat) had no effect on SBP in Wistar–Kyoto rats (WKY), despite significantly decreased catecholamine levels, TH activity, and TH protein levels. These findings suggest that peripheral systemic injection of TH AS ODN may be effective as hypotensive therapy in SHR.
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