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The Influence of the Amount of Crosslinker in the Electrophoretic Mobility of Cytoskeletal Proteins in Polyacrylamide Gels

 

作者: A. Alcover,   J. Avila,  

 

期刊: Analytical Letters  （Taylor Available online 1985）
卷期: Volume 18, issue 9  

页码: 1157-1165

 

ISSN:0003-2719

 

年代: 1985

 

DOI:10.1080/00032718508069107

 

出版商: Taylor & Francis Group

 

关键词: electrophoresis;vimentin;keratin;tubulin

 

数据来源: Taylor

 

摘要:

The influence in the amount of crosslinker (methylenebisacrylamide) on the mobility of peptides during polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate has been tested. A drastic change in the mobility, dependent on the ratio crosslinker: acrylamide used, was found for keratins and vimentin which could invert their mobility with respect to other polypeptides.

 

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