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A Critical Review of Typing Methods for Candida albicans and Their Applications

 

作者: HunterPaul R,  

 

期刊: Critical Reviews in Microbiology  (Taylor Available online 1991)
卷期: Volume 17, issue 6  

页码: 417-434

 

ISSN:1040-841X

 

年代: 1991

 

DOI:10.3109/10408419109115206

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

AbstractDuring the 1980s, a large number of typing methods for the strain differentiation ofCandida albicanswere described in the literature. Although these methods have been based on a variety of physiological and genetic markers, none is ideal. This review discusses the characteristics of an ideal typing method in terms of its typability, reproducibility, and discriminatory power. Ways of determining these characteristics are presented so that the available typing methods forCandida albicanscan be objectively compared. Available typing methods forC. albicansinclude serotyping, morphotyping, resis-totyping, biotyping, and killer yeast typing. Electrophoretic methods include immunoblotting, isoenzyme analysis, analysis of DNA restriction fragment length polymorphism, karyotyping, and the use of DNA probes. The application of these methods to epidemiological research, the investigation of outbreaks of disease, and die study of virulence is described. The potential impact of the phenomenon of phenotypic switching on the reproducibility of these typing methods is discussed. It is concluded that many of the available typing methods have not been adequately assessed by their developers and that several have only poor discriminatory power or reproducibility.

 

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