3-amino-triazole effects on the eye of young and adult rabbits in the presence and absence of hydrogen peroxide
作者:
BirnbaumDavid,
CsukasStephen,
CostaridesAnastasios,
ForbesElizabeth,
GreenKeith,
期刊:
Current Eye Research
(Taylor Available online 1987)
卷期:
Volume 6,
issue 12
页码: 1403-1414
ISSN:0271-3683
年代: 1987
DOI:10.3109/02713688709044504
出版商: Taylor&Francis
数据来源: Taylor
摘要:
3-aminotriazole (3AT) is known to reduce catalase levels in ocular tissues when given intravenously or orally. Rabbits were given either 4 ml/kg of a 3M solution of 3AT intravenously or a 2% solution as drinking fluid. Intravenous 3AT administration was followed at 4 hrs by an intracameral injection of hydrogen peroxide (H2O2) to give an aqueous humor concentration of 3.2 mM in young (4–6 weeks of age) and a 3.3 mM in adult (6 months of age) rabbits. Tissues were taken for microscopy at either 6 or 24 hours after intracameral H2O2. Neither oral nor intravenous 3AT alone in adult rabbits, or intravenous 3AT in young rabbits, had any effect on either iris, ciliary process, or corneal endothelial morphology. After oral 3AT in adult rabbits, H2O2caused highly edematous ciliary processes with dilated vessels; corneal endothelial cells were swollen. Previous studies in adult and young rabbits have shown that intracameral H2O2alone caused few morphological changes in young, but marked changes in the adult that correlated with the 35 to 50% lower catalase levels found in iris and corneal endothelium, respectively, in adult ocular tissues. Young rabbits pre-treated with intravenous 3AT, when examined at 6 and 24 hours after intracameral H2O2showed swollen ciliary processes, vessel dilation, alteration of the pigment epithelium and corneal endothelial damage. In non 3AT–treated young rabbits, H2O2caused only minor morphological changes. In adult animals at 6 and 24 hours after intracameral H2O2the ciliary processes were edematous in the absence of 3AT; after intravenous 3AT and intracameral H2O2the changes were even more marked, with very severe swelling of ciliary processes and corneal endothelial damage. It is apparent that the decrease in catalase caused by 3AT allows H2O2to induce damage even in young animals where it usually does not induce morphological changes. In adult animals, the effects of H2O2are enhanced in the presence of 3AT.
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