EFFICIENT EXTRACTION OF PACLITAXEL AND RELATED TAXOIDS FROM LEAF TISSUE OFTAXUSUSING A POTABLE SOLVENT SYSTEM
作者:
RaymondE. B. Ketchum,
JudyV. Luong,
DonnaM. Gibson,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 11
页码: 1715-1732
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101762
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Leaves ofTaxus x mediaandTaxus brevifoliawere examined for their potential use as a source of paclitaxel and related taxoids. Various solvent systems were compared for their efficacy in the extraction of paclitaxel, as well as the extraction of contaminating residue. A method of extraction has been developed that enriches paclitaxel by over 500-fold using only potable solvents in combination with solid phase extraction, thus avoiding the use of chlorinated hydrocarbon solvent systems. This solvent system can be used on fresh leaf material, eliminating the need for drying material before extraction, and preventing degradation of the taxoids during the drying process. Using these methods, paclitaxel constituted 0.01 to 0.02% of the dry weight of the leaf tissue fromTaxus x media, and 0.04% from dried leaves ofTaxus brevifolia.
点击下载:
PDF (157KB)
返 回