The Mass Uptake of Cholesterol Ester from Low Density Lipoproteins by Cultured Smooth Muscle and Adventitial Cells of Human Aortas
作者:
SYED ALAM,
KENNETH SOLEN,
DON LAYMAN,
MATTHEW RIDDLE,
WILLIAM CONNOR,
期刊:
Circulation Research
(OVID Available online 1980)
卷期:
Volume 47,
issue 3
页码: 374-383
ISSN:0009-7330
年代: 1980
出版商: OVID
数据来源: OVID
摘要:
Cultured human smooth muscle and adventitial cells were incubated with human serum and low density lipoprotein (LDL) to study the Uptake and accumulation of cholesterol ester from exogenous LDL. The cellular total cholesterol varied with the amount of LDL cholesterol in the medium. The cholesterol ester content increased 4-fold after 2 hours of incubation. A 6-fold rise occurred by 24 hours and continued to 72 hours. The cholesterol ester of the adventitial cells was markedly depleted by incubation with abetalipoproteinemic serum or with a lipid-depleted plasma fraction. By the use of14C-labeled LDL free cholesterol in the incubation medium, we calculated that some 70-80% of the total accumulated cholesterol ester after 24 hours of incubation was derived from LDL cholesterol ester, and only 20-30% was synthesized by the cells. These studies demonstrated conclusively that human cells greatly increase their cholesterol ester mass after incubation with LDL.Circ Res 47: 374-383, 1980
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