Description of a BHK/21 Cell Line Persistently Infected with Junin Virus: Its Use in Diagnostic Procedures
作者:
Guadalupe Carballal,
Patricio M. Cossio,
Adriana Rabinovich,
José Oubiña,
Roberto M. Arana,
期刊:
Intervirology
(Karger Available online 1980)
卷期:
Volume 14,
issue 3-4
页码: 173-179
ISSN:0300-5526
年代: 1980
DOI:10.1159/000149180
出版商: S. Karger AG
关键词: BHK/21 cells;Argentine hemorrhagic fever;Immunofluorescence;Carrier state;Persistent infection
数据来源: Karger
摘要:
A BHK/21 cell line persistently infected by an arenavirus is described. During four consecutive passages, 30–45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both Argentine hemorrhagic fever convalescent sera and sera from animals immunized with Junin virus. Virus isolated from the cells killed suckling mice but not adult mice and protected guinea pigs against further challenge with the virulent prototype strain of Junin virus. Neutralization tests showed that the virus isolated from the cells was neutralized by anti-Junin virus antisera. The usefulness of this cell line in rapid immuno-fluorescent serological procedures is describe
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