Rats of a normal selenium status were fed diets based on fish (rainbow trout) as the main protein source. A 4-week experiment with three dietary groups (low fish selenium, high fish selenium and selenite supplementation) was performed, and the selenium absorption, excretion and retention were recorded. Samples of blood serum, liver, kidneys, testes, hairs, spleen, lungs, heart, brain and skeletal muscle were collected for analysis of selenium. Glutathione peroxidase activity was measured in the blood serum. A selenium supplementation of approximately 1 mg/kg (high fish selenium and selenite group) yielded a selenium retention of only 7 % of the intake, while in the group with a dietary selenium concentration of approximately 0.1 mg/kg (low fish selenium group) the selenium retention was 50%, resulting in almost the same absolute selenium retention in all three groups. The liver and kidneys showed the highest accumulations of selenium, reflecting the participation of these organs in the excretion of surplus selenium. The highest relative uptake of selenium was recorded in the testes, which increased equally in all dietary groups. The selenium concentration in the other tissues investigated, as well as the glutathione peroxidase activity in the blood serum responded little to the selenium supplementations. These results showed that the selenium levels normally found in fish were sufficient to satisfy the need for this element in rats of a good selenium status, and that inorganic selenite was absorbed and excreted at a high rate already after 1 week.