Abstract.Among samples collected from nineteen localities in Papua New Guinea, we have identified six species within theAnopheles punctulatuscomplex of mosquitoes, by means of cellulose acetate allozyme electrophoresis.An.punctulatusDönitzsensu strictowas collected from seven villages in the Madang area and from Buksak, Sausi Mission and an area 18 km SW of Tari;An.koliensisOwen from eight villages in the Madang area, from Popondetta and Brown River near Karema; andAn.farautiNo. 1 from ten coastal areas including Madang, Lorengau, Popondetta, Port Moresby, Rabaul and Wewak. Three newly recognized species, reported here for the first time, are designated asAn.farautiNo. 4 from Gonoa and Hudini, Madang area;An.farautiNo. 5 from Ketarabo near Goroka; andAn.farautiNo. 6 from Hiwanda near Tari. Three other known members of the complex,An.clowiRozeboom&Knight,An.farautiNo. 2 (Bryan, 1973) andAn.farautiNo. 3 (Mahon&Meithke, 1982) were not detected in Papua New Guinea. Problems arising with morphological characters for the identification of species in this group are discussed