Genome size variations are related to X‐chromosome heterochromatin polymorphism inArvicanthissp. from Benin (West Africa)
作者:
Silvia Garagna,
MariaVittoria Civitelli,
Nicola Marziliano,
Riccardo Castiglia,
Maurizio Zuccotti,
CarloAlberto Redi,
Ernesto Capanna,
期刊:
Italian Journal of Zoology
(Taylor Available online 1999)
卷期:
Volume 66,
issue 1
页码: 27-32
ISSN:1125-0003
年代: 1999
DOI:10.1080/11250009909356233
出版商: Taylor & Francis Group
关键词: Genome size;Heterochromatin;Sex‐chromosomes;Arvicanthis
数据来源: Taylor
摘要:
The African grass rat,Arvicanthis sp., from Benin shows sex‐chromosome polymorphism for the presence in the karyotype of three X‐chromosome forms: subtelocentric (Xst), submetacentric (Xsm), and metacentric (Xm). Here we report statistically significant differences of diploid genome size (g.s.) among individuals, from 6.22 ± 0.06 pg to 6.80 ± 0.10 pg, depending on the X‐chromosome form. The evaluation of the total and X‐chromosome heterochromatin content alone (Xst: 2.16 ± 0.22%; Xsm: 5.02 ± 0.22%; Xm: 6.54 ± 0.28%), supports this suggestion. DNA base‐pair preferential staining revealed that heterochromatin of both autosome and sex chromosomes is AT‐rich. Digestion of genomic DNA with sixteen restriction enzymes produced DNA bands representing the repetitive fraction of the genome. When these were used as probes for fluorescencein situhybridization (FISH), two hybridization patterns were found: a) FISH positive signals localized in the pericentromeric areas of every chromosome of the complement and b) chromosome specific signals co‐localizing with both the pericentromeric regions of every chromosome and the heterochromatic regions of Xsm and Xm, but not on the Xst chromosome. FISH performed with a (TTAGGG)n probe revealed a telomere‐only pattern.
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