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Purification and properties of ginger chlorotic fleck virus

 

作者: J. E. THOMAS,  

 

期刊: Annals of Applied Biology  (WILEY Available online 1986)
卷期: Volume 108, issue 1  

页码: 43-50

 

ISSN:0003-4746

 

年代: 1986

 

DOI:10.1111/j.1744-7348.1986.tb01964.x

 

出版商: Blackwell Publishing Ltd

 

数据来源: WILEY

 

摘要:

SUMMARYA previously undescribed isometric virus, named ginger chlorotic fleck virus (GCFV), was detected in ginger (Zingiber officinale) imported into Australia from a number of countries. The geographical distribution of the virus is uncertain, but is thought to include India, Malaysia and Mauritius. The virus apparently does not occur in Australian commercial ginger plantings.The virus has isometric particlesc. 30 nm in diameter, with a sedimentation coefficient of 111 S, and was readily purified from infected ginger with yields of 50–90 mg/kg leaf tissue. Purified preparations contained a major species of single‐stranded RNA of mol. wt 1.50 × 106and a major coat protein species of mol. wt 29.0 × 103. At pH 7, the particles formed a single zone in both caesium chloride and caesium sulphate gradients, with buoyant densities of 1.355 g cm‐3(fixed virus) and 1. 297 g cm‐3(unfixed virus), respectively. The virus particles migrated as two electrophoretic components and were labile when treated with 10 mM EDTA, 1 M NaCI, 10 mM tris pH 8.25 or when negatively stained with potassium phosphotungstate. GCFV was mechanically transmitted only to ginger, and was not transmitted by the aphidsMyzus persicae. Pentalonia nigronervosa, Rhopalosiphum maidisorR. padi. Possible affinities of GCFV with the sobemo‐virus group are discussed.The present cryptogram of GCFV isR/l:1.5

 

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