EFFECTS OF DOSE FRACTIONATION ON ULTRAVIOLET SURVIVAL OFESCHERICHIA COLI
作者:
Walter Harm,
期刊:
Photochemistry and Photobiology
(WILEY Available online 1968)
卷期:
Volume 7,
issue 1
页码: 73-86
ISSN:0031-8655
年代: 1968
DOI:10.1111/j.1751-1097.1968.tb05831.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
Abstract—Exposure ofE. coliB/r and B at low average dose rates of u.v. radiation (2537 Å), produced either by fractionated doses or by continuous irradiation at a very low dose rate (80 ergs/mm2/hr), results in much increased survival compared to single exposure at high dose rate. This increase is attributed to repair taking place during the irradiation period. The effect is small in the repair‐deficient strainsE. coliB8‐1_, and Csyn‐, and is absent in phage T1 and T4, which cannot undergo repair in the extracellular state. However, the prolonged time available for repair in these experiments accounts for only a very minor part of the increase in survival. The principal factor apparently is that the number of lesions present at any time remains relatively low. Presumably complete repair, not only the excision step, can occur in buffer during the irradiation period. This interpretation is supported by experiments in which cells were exposed to combinations of highly fractionated irradiation and single‐dose irradiation. We therefore propose that mutual interference in repair, possibly by overlapping of repair regions in complementary DNA strands, reduces considerably the repair efficiency if many lesions are present. This hypothesis explains the ‘shouldered’ survival curves of B/r and possibly otherE. colistrains as due to decreasing repair efficiency with incre
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