Molecular Cloning of a Novel G Protein‐Coupled Receptor Related to the Opiate Receptor Family
作者:
Jean E. Lachowicz,
Yong Shen,
Frederick J. Monsma,
David R. Sibley,
期刊:
Journal of Neurochemistry
(WILEY Available online 1995)
卷期:
Volume 64,
issue 1
页码: 34-40
ISSN:0022-3042
年代: 1995
DOI:10.1046/j.1471-4159.1995.64010034.x
出版商: Blackwell Science Ltd
关键词: G protein‐coupled receptor;Opiate receptor;Polymerase chain reaction;Neuropeptide
数据来源: WILEY
摘要:
Abstract:A cDNA clone encoding a novel G protein‐linked receptor was isolated from a rat cerebral cortex cDNA library using a polymerase chain reaction‐amplified cDNA fragment as a probe. This 2.4‐kb clone encodes a 367 amino acid protein with seven putative transmembrane spanning domains. The protein is highly homologous to the cloned μ, δ, and κ opioid receptors and shares with them structural features such as three glycosylation sites in the amino terminus, a cyclic AMP‐dependent kinase phosphorylation site in the third cytoplasmic loop, an aspartic acid residue in the second transmembrane domain, and a palmitoylation site on the intracellular carboxy terminus. The receptor is also homologous with members of the somatostatin receptor family, yet it binds neither opiate nor somatostatin ligands. Northern blot analysis reveals two transcripts of 3.2 and 7.6 kb that are predominantly expressed in the cerebral cortex and hypothalamus. In situ hybridization analysis also shows a high abundance of mRNA in the cerebral cortex, hippocampus, amygdala, hypothalamus, thalamus, and dorsal raphe nuclei. It is suggested that the endogenous ligand for this receptor may represent a novel neuropeptide that may be closely related to the opiate pept
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