Insulin-Like Growth Factor-1 Stimulates Proliferation of Myeloid FDC-P1 Cells Overexpressing the Human Colony-Stimulating Factor-1 Receptor
作者:
BouretteRoland P.,
Von RüdenThomas,
BallmerKurt,
MorléFrançois,
PaulJean,
MouchiroudGuy,
期刊:
Growth Factors
(Taylor Available online 1992)
卷期:
Volume 7,
issue 4
页码: 315-325
ISSN:0897-7194
年代: 1992
DOI:10.3109/08977199209046414
出版商: Taylor&Francis
关键词: FDC-P1 cells;CSF-1 receptor;IGF-I receptor
数据来源: Taylor
摘要:
AbstractRetrovirally expressed human CSF-1 receptor can induce CSF-1-dependent growth of IL-3-dependent hemopoietic cells FDC-P1. Here we show that expression of the human CSF-1 receptor also allowed FDC-P1 cells to grow in response to Insulin-like Growth Factor-1 (IGF-I). The authentic receptor for IGF-I was identified by affinity cross-linking and binding analysis on both control (infected with aneovector) and CSF-1 receptor expressing FDC-P1 cells. DNA and RNA analysis of these cells and of five clones of IGF-I responsive cells demonstrated that the IGF-I receptor gene was not rearranged nor was it abnormally expressed in IGF-I responsive cells. These results suggest that myeloid cells over-expressing CSF-1R (c-fmsprotooncogene product) might have a proliferative advantage over normal myeloid cells in a physiological situation, independently of the presence of CSF-1 or the capacity of the cells to respond to CSF-1. This would indicate a possible role forc-fmsin human neoplasia.
点击下载:
PDF (1079KB)
返 回