Serial Thick, Frozen, Gallocyanin Stained Sections of Human Centrl Nervous System
作者:
HeinsenH.,
期刊:
Journal of Histotechnology
(Taylor Available online 1991)
卷期:
Volume 14,
issue 3
页码: 167-173
ISSN:0147-8885
年代: 1991
DOI:10.1179/his.1991.14.3.167
出版商: Taylor&Francis
关键词: CNS;comparative anatomy;cytoarchitectonics;neuroimaging;neuropathology;Nissl stain;quantitative
数据来源: Taylor
摘要:
AbstractA rapid method for macroscopic and microscopic investigation of human CNS is proposed. After formalin fixation, gelatin or agarose embedding, and cryoprotective treatment, frozen human spinal cords, brainstems, or hemispheres can be serially cut into 0.7 mm thick slices. Stained with gallocyanin- chromalum, these slices facilitate cytoarchitectonic, neuropathologic, and quantitative examination. Regions of interest from parallel formalin-stored unstained slices can be embedded into paraffin and stained by any immunocytologic and histologic stain compatible with formalin fixation and paraffin embedding. (The J Histotechnol14:167, 1991)
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