Quick and Simple Determination of Dihydroergotamine by High-Performance Liquid Chromatography
作者:
EsmailM. Niazy,
AbdullaM. Molokhia,
AbubakrS. El-gorashi,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 10
页码: 1833-1843
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808066350
出版商: Taylor & Francis Group
关键词: Dihydroergotamine;Analysis;LC;Plasma Levels.
数据来源: Taylor
摘要:
A simple and rapid liquid chromatographic assay method using a fluorescence detector for quantitation of dihydroergotamine in plasma without extraction was developed. After precipitating the protein with acetonitrile, the supernatant liquid was directly injected for analysis. Chromatographic separation was achieved on C18reversed phase column and the mobile phase was the isocratic mixture of methanol, acetonitrile and glycine buffer (0.5:3.5:6.0). With this eluting solvent the drug and its internal standard were well separated from the interference of the plasma sample. The average recovery of dihydroergotamine from 6 replicate samples of different concentrations (5-30 ng/ml) were 92.2 ± 3.37%. The minimum amount of dihydroergotamine detectable by this method was 2 ng/ml of sample.
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