SummaryThe complete nucleotide sequence of theCorynebacterium glutamicum hom‐thrBoperon has been determined and the structural genes and promoter region mapped. A polypeptide of Mr46136 is encoded byhomand a polypeptide of Mr, 32618 is encoded bythrB.Both predicted protein sequences show amino acid sequence homology to their counterparts inEscherichia coliandBacillus subtilis.The promoter region has been mapped by S1‐nuclease and deletion analysis. Located between −88, RNA start site and −219 (smallest deletion clone with complete activity) are sequence elements similar to those found inE. coliandB. subtillspromoters. Although there are no obvious attenuator‐like structures in the 5′‐untranslated region, there is a dyad‐symmetry element, which may act