Development and Application of a Genotypic AZT Resistance AssayQuantitative Assessment of the Resistance Profile of Clinical Samples and the Relative Predictive Ability of a Resistance Index
作者:
Tassos Kyriakides,
Robert Heimer,
期刊:
JAIDS Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 2001)
卷期:
Volume 28,
issue 3
页码: 211-220
ISSN:1525-4135
年代: 2001
出版商: OVID
关键词: HIV drug resistance/resistance mutations;Resistance index;Relative predictive ability
数据来源: OVID
摘要:
ObjectivesTo develop and validate a rapid, genotypic, quantitative AZT resistance assay, and to evaluate the predictive ability of a resistance index.MethodsAZT resistance profiles of paired samples from HIV-infected patients were determined by a ligase chain reaction (LCR) assay. AZT resistance levels and surrogate markers of HIV disease progression (viral load and CD4 counts) were used to compare AZT-naive and AZT-experienced patients. The ability of a “mutant/wild-type HIV-1 quasi-species” index to predict viral load was assessed.ResultsAZT resistance, evident at baseline in both AZT-experienced and AZT-naive patients, increased over 6 months of treatment. The resistance profile of AZT-naive patients differed from that of AZT-experienced patients (p< .05); viral load and CD4 counts were similar. The relative predictive ability (for subsequent viral load) of the resistance index was similar to or higher than that of baseline viral load or CD4 count.ConclusionsThis assay used to detect AZT resistance could be adapted for use with other antiretrovirals. The predictive ability of the proposed resistance index was equal to or surpassed that of viral load and CD4 count, lending further support to the use of resistance assays in selecting drug regimens both before and during antiretroviral therapy.
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