Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate
作者:
Robert M. Pratt,
Robert I. Grove,
William D. Willis,
期刊:
Teratogenesis, Carcinogenesis, and Mutagenesis
(WILEY Available online 1982)
卷期:
Volume 2,
issue 3‐4
页码: 313-318
ISSN:0270-3211
年代: 1982
DOI:10.1002/1520-6866(1990)2:3/4<313::AID-TCM1770020312>3.0.CO;2-C
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: secondary palate;human;mesenchyme cell;growth inhibition
数据来源: WILEY
摘要:
AbstractMesenchymal cells from the prefusion human embryonic palate have been established in culture and can be grown in either a serum‐free hormone‐supplemented medium or a serum‐containing medium. The growth of these cells is quite rapid in culture and inhibited in a dose‐dependent manner by most teratogens thus far tested, such as dexamethasone. These cells are highly sensitive to a variety of DNA synthetic and mitotic inhibitors. The responses of these cells are complementary to the ovarian tumor cell attachment assay of Braun et al [1, and in this volume]. When used in conjunction with the tumor cells, the overall reliability is greater than 90% with only one false‐negative, al
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