Opioids activate G proteins in REM sleep‐related brain stem nuclei of rat
作者:
M Capece,
Helen Baghdoyan,
Ralph Lydic,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 13
页码: 3025-3028
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: DAMGO;In vitroautoradiography;Laterodorsal tegmental nucleus;Opioid receptors;Pedunculopontine tegmental nucleus;Pontine reticular formation
数据来源: OVID
摘要:
MU opioid receptors within the pontine reticular formation contribute to opioid-induced rapid eye movement (REM) sleep inhibition. Mu receptors are coupled to guanine nucleotide binding (G) proteins and this study tested the hypothesis that th μ opioid agonist [D-Ala2,N-Me-Phe4,Gly-ol5]enkephalin (DAMGO) would activate G proteins in rat brain stem nuclei known to regulate REM sleep.In vitroautoradiography of DAMGO-stimulated [35S]GTPγS binding showed that, compared with basal [35S]GTPγS binding, DAMGO significantly increased G protein activation in the nucleus pontis oralis (56.2%), nucleus pontis caudalis (57.3%), laterodorsal tegmental nucleus (75.8%), pedunculopontine tegmental nucleus (72.4%), nucleus locus coeruleus (77.2%) and dorsal raphe nucleus (73.4%). DAMGO stimulation of [35S]GTPγS binding in nuclei regulating REM sleep suggests that opioid-induced REM sleep inhibition involves activation of G proteins.
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