Detection of Anabolic Steroids By Gc/Sim/Ms With Trifluoroacetylation in Equine Plasma and Urine
作者:
Man Ho Choi,
Jin Young Kim,
Bong Chul Chung,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 7
页码: 1313-1322
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542899
出版商: Taylor & Francis Group
关键词: Anabolic Steroids;GC/MS;Trifluoroacetylation;Equine
数据来源: Taylor
摘要:
A method to detect three anabolic steroids (boldenone, nandrolone and mesterolone) is presented. The anabolic steroids are isolated from equine plasma and urine by extraction with diethyl ether and C18Sep-Pak cartridge adsorption, respectively. The extracts obtained were derivatized with trifluoroacetyl anhydride and analyzed by GC/SIM/MS. The selected ion monitoring (SIM) mode was applied to increase the sensitivity and, when possible, the higher m/z ions were selected to improve identification. Stability of derivatives was good and compounds having hydroxy and conjugated ketone groups produced trifluoroacetyl ester derivatives that were also stable. Repeatability of the chromatographic analysis was evaluated on the basis of area repeatability, and the coefficient of variation obtained was lower than 4.4. The detection limit was 1 and 5 ng/ml for all the anabolic steroids studied in equine plasma and urine, respectively.
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