A quantitative chemometric approach, iterative target transformation factor analysis (ITTFA), was applied to the simultaneous determination of mixtures of four food colorants (Amaranth, Sunset Yellow. Tartrazine and Ponceau 4R) by adsorptive voltammetry. The conventional and first-derivative voltammograms of the quaternary mixtures were used to perform the optimization of the calibration procedure by the ITTFA models. The proposed method was applied satisfactorily to the determination of a set of synthetic quaternary mixtures of colorant in phosphate/citric acid buffers (pH 5.7) and acceptable results were obtained. No significant advantages were found with the application of derivative technique in this voltammetric work.