Protoplast Cultures from Cell Suspensions ofDaucus carota
作者:
ANITA WALLIN,
TAGE ERIKSSON,
期刊:
Physiologia Plantarum
(WILEY Available online 1973)
卷期:
Volume 28,
issue 1
页码: 33-39
ISSN:0031-9317
年代: 1973
DOI:10.1111/j.1399-3054.1973.tb01147.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
AbstractProtoplasts, enzymatically isolated from cell suspension cultures ofDaucus carota, have been grown in small Petri‐dishes. After enzyme treatment and washing the protoplasts were plated in agar media. Growth and divisions were viewed through the bottom of the Petri‐dishes with a light microscope. Different osmotic stabilizers were tested with respect to their ability to promote wall formation and growth of the protoplasts. Combinations of sucrose, sorbitol and “Modopeg” gave the best results. Electron micrographs of cultured protoplasts revealed normal as well as abnormal nuclear con
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