Electron-Microscopic Localization of Baboon Acrosomal Antigens Using Antisperm Monoclonal Antibody
作者:
P.M. Gichuhi,
M.A. Isahakia,
期刊:
Cells Tissues Organs
(Karger Available online 1992)
卷期:
Volume 144,
issue 2
页码: 178-183
ISSN:1422-6405
年代: 1992
DOI:10.1159/000147304
出版商: S. Karger AG
关键词: Acrosomal antigens;Sperm;Monoclonal antibody;Immunogold labelling;Baboon
数据来源: Karger
摘要:
A sperm antigen corresponding to baboon sperm monoclonal antibody 1A9 was localized in the testis and ejaculated sperm in this animal, using the immunofluorescence technique and immunogold labelling. Immunohistochemical studies of the baboon testis showed that the antigenic determinant was localized in the late spermatid cells and spermatozoa close to the seminiferous tubules. Immuno-fluorescence studies indicate that the protein was localized on the acrosome region of ejaculated baboon sperm. At the electron-microscopic level, gold particles indicative of the presence of this determinant recognized by 1A9 monoclonal antibody were detected on the inner acrosomal region of ejaculated baboon sperm.
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