A New Bullous Pemphigoid Antigen
作者:
S. Fujiwara,
K. Kohno,
A. Iwamatsu,
H. Shinkai,
期刊:
Dermatology
(Karger Available online 1994)
卷期:
Volume 189,
issue 1
页码: 120-122
ISSN:1018-8665
年代: 1994
DOI:10.1159/000246950
出版商: S. Karger AG
关键词: Bullous pemphigoid antigen;Molecular cloning;450-kD antigen
数据来源: Karger
摘要:
Previously we reported a case of subepidermal blistering disease, which resembled bullous pemphigoid clinically. An immunoblot analysis revealed that the patient’s serum reacted exclusively with a 450-kD epidermal polypeptide. The 450-kD antigen was also expressed in human keratinocytes and a human squamous carcinoma (HO 1-u-1 or A431) cell line. Using the patient’s serum, cDNA clones in a bacteriophage λgt11 library derived from human foreskin keratinocytes were immune-screened. One positive clone was isolated from 2 million clones. Northern blot analysis showed that the inserted part of this clone hybridized with a more than 12-kb mRNA species derived from HO 1-u-1 cells. The deduced amino acid sequence had no homology with any other human compo
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