The purpose of this study was to provide a reference for xeroradiographic and conventional radiographic anatomy of the great horned owl (Bubo virginianus) as a representative of the avian Order Strigiformes. The head, body, wing, and pelvic limb of three adult birds were radiographed using xeroradiographic and conventional radiographic techniques. Twelve xeroradiographs and their corresponding conventional radiographs were selected, and the xeroradiographs labeled to illustrate normal anatomy. Selected views of the barn owl (Tyto alba) were included to illustrate significant differences between this and other species of owls.