The Dendritic Extent of Motoneurons in Frog Brachial Spinal Cord: A Computer Reconstruction of HRP-Filled Cells
作者:
Bennett M. Rosenthal,
William L.R. Cruce,
期刊:
Brain, Behavior and Evolution
(Karger Available online 1985)
卷期:
Volume 27,
issue 2-4
页码: 106-114
ISSN:0006-8977
年代: 1985
DOI:10.1159/000118724
出版商: S. Karger AG
关键词: Computer-microscope system;Dendrites;Motoneurons;Frog spinal cord;Sholl analysis
数据来源: Karger
摘要:
A lateral and a medial motoneuron in the brachial spinal cord of the leopard frog, Rana pipiens, were labeled by horseradish peroxidase applied to the ventral root. Their dendritic trees were traced, analyzed, and plotted using a computer-microscope system. Some dendrites of the medial motoneuron crossed the midline of the spinal cord, but no dendrites of the lateral motoneuron crossed the midline. Nevertheless, the total dendritic length of the lateral motoneuron exceeded that of the medial motoneuron. The peak number of dendritic branch segments of the medial motoneuron was located at a greater distance from its soma than that of the lateral motoneuron. Three-dimensional reconstruction and rotation of the dendritic trees revealed that the dendrites of the medial motoneuron had a greater rostrocaudal extent than those of the lateral motoneuron. When compared to reports of Golgi-impregnated motoneurons our results suggest that the HRP technique labels dendrites more completely. However, use of the HRP technique may introduce greater errors in the subsequent measurement of dendritic segments due to nonuniform tissue shrinkage.
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