A procedure for stabilization of cytopherometersfor immunological applications
作者:
Ulf D. Koenig,
Lothar Bongartz,
A. Burak Kozan,
期刊:
ELECTROPHORESIS
(WILEY Available online 1982)
卷期:
Volume 3,
issue 1
页码: 49-52
ISSN:0173-0835
年代: 1982
DOI:10.1002/elps.1150030109
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA device commonly used for cell electrophoresis is the cytopherometer. Its application in an immunological tumor test, however, pointed out many shortcomings and instabilities. In order to use cytopherometers in cellular immunology, a procedure was worked out to stabilize this instrument. By enlarging the electrode chambers, using a simple and efficient buffer in all compartments, coating the electrophoresis chamber, and by following a set of electrophoretic parameters (current, temperature,etc.), an intraassay variance of 1.8 % and an interassay variance of approximately 1 % were reached.
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