Tillage practices impart physical changes to the solum that may alter patterns of nutrient availability in soil profiles. The soil solution composition may be indicative of altered patterns of nutrient availability. Soil solution compositional analysis was used to compare the effects of 7 years continuous no-tillage (NT) vs. conventional tillage (CT) management of corn (Zea maysL.), and differing times of N fertilization, on solute distribution in a Loring (fine-silty, mixed, thermic Typic Fragiudalf) soil subjected to continuous CT for >20 years. Tillage plots received 168 kg N ha−1as NH4NO3, broadcast either at planting (Early) or 5 weeks after planting (Late). Soil solutions obtained by vacuum displacement for three sampling dates (3 April, 8 May, and 24 June) were analyzed for pH, electrical conductance (EC), dissolved organic C (DOC), and total concentrations of K, Na, Ca, Mg, NO3, Cl, SO4, and Al (24 June only). Tillage and time of N application frequently interacted to influence solute distribution in the soil profile. Comparisons of CT Early and CT Late with NT Early and NT Late, respectively, consistently indicated higher EC, NO3, Ca, and Mg for subsoils (>30 cm) sampled under CT. The imposition of NT on a soil that had been in long-term continuous CT resulted in altered patterns of nutrient distribution as reflected in the composition of soil solution sampled throughout the growing season