On the Phospholipid Metabolism of Glial Cell Primary Cultures.
作者:
Brigitte Witter,
Johannes Gunawan,
Hildegard Debuch,
期刊:
Journal of Neurochemistry
(WILEY Available online 1983)
卷期:
Volume 40,
issue 1
页码: 64-69
ISSN:0022-3042
年代: 1983
DOI:10.1111/j.1471-4159.1983.tb12653.x
出版商: Blackwell Publishing Ltd
关键词: Primary cultures;Glial cells;Phospholipids;Ether lipids
数据来源: WILEY
摘要:
Abstract:Primary cultures were prepared from newborn rat brain. After 16‐18 days, they consisted mainly of mature and immature astrocytes and oligodendrocytes, as judged by immunohistochemistry. To study the metabolism of ethanolamine glycerophospholipids, the cells were incubated with 1‐[1‐3H]alkyl‐sn‐glycero‐3‐phosphoethanolamine (1‐alkyl‐GPE), for 1–20 h. Five main products were formed: 1‐alkyl‐2‐acyl‐GPE; 1‐alkyl‐2‐acyksn‐glycero‐3‐phosphocholine (1‐alkyl‐2‐acyl‐GPC); 1‐alkenyl‐2‐acyl‐GPE (ethanolamine plasmalogen); 1‐alkenyl‐2‐acyl‐GPC (choline plasmalogen); and 1‐alkyl‐glycerol. Acylation of the substrate was the main reaction during the first 3 h of incubation, whereas desaturation to plasmaiogen reached a maximum after 12 h. Greater amounts of radioactivity were observed in the phosphatidylcholine fraction after longer incubation times. Only small amounts of choline plasmalogen were observed. The phosphatidylethanolamine fraction consisted of 26.5% diacyl‐, 27.5% alkyl‐acyl‐, and 46.0% alkenyl‐acyl‐ compounds, whereas the corresponding data for the phosphatidylcholine fraction were 78.5, 16.4, and 5.1%, respectively, after 20 h of inc
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