Golgi Method without Osmium Tetroxide for the Study of the Central Nervous System
作者:
GonzalezIgnacio,
TapiaGuadalupe,
FeriaAlfredo,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 5
页码: 288-296
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110037
出版商: Taylor&Francis
关键词: Golgi stain;osmium tetroxide;formaldehyde;central nervous system
数据来源: Taylor
摘要:
A variant Golgi technique was developed that consisted of substituting osmium tetroxide with formaldehyde as the initial fixative in intracardiac perfusion, along with the addition of glacial acetic acid to the chromating fluid. This procedure avoids disposal of dangerous waste substances into the environment. Other advantages include 1) reduction of cost, danger to lab workers, and risk of disruption of the tissue slices during their handling by eliminating the osmium tetroxide, 2) clear tissue background, 3) greater quantity of impregnated neurons than in the classical procedure, with distinct morphological details easily identified even in gross sections and 4) reduction in processing time.
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