Characterization of Neurophysin in the Anterior Pituitary Gland of Sheep
作者:
Wayne B. Watkins,
Lloyd G. Moore,
Joachim Spiess,
期刊:
Neuroendocrinology
(Karger Available online 1988)
卷期:
Volume 47,
issue 2
页码: 142-148
ISSN:0028-3835
年代: 1988
DOI:10.1159/000124904
出版商: S. Karger AG
关键词: Neurophysin;Rat anterior pituitary gland;Corticotrophs;Sequence analysis
数据来源: Karger
摘要:
Portions of sheep anterior pituitary lobe tissue were extracted under acid conditions and assayed for the two neurophysins (oN-III and oN-I/II) by radioimmunoassay. In all tissues examined, oN-III and oN-I/II immunoreactivi-ties were detected. Using a combination of isoelectric focusing and polyacrylamide gel electrophoresis, the neurophysins of the anterior pituitary gland behaved like oN-III and oN-I/II. oN-III of the anterior pituitary was purified by affinity chromatography and high-performance liquid chromatography. This corticotroph oN possessed an amino acid analysis similar to that of oN III and an N-terminal amino acid sequence, including residues 1–24, identical to that of authentic oN-III. These findings support the work of others who have identified neurohypophysial hormones in the anterior pituitary glan
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