首页   按字顺浏览 期刊浏览 卷期浏览 Thionin Staining of Paraffin and Plastic Embedded Sections of Cartilage
Thionin Staining of Paraffin and Plastic Embedded Sections of Cartilage

 

作者: BulstraS. K.,   DrukkerJ.,   KuijerR.,   BuurmanW. A.,   LindenA. J. van der,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1993)
卷期: Volume 68, issue 1  

页码: 20-28

 

ISSN:1052-0295

 

年代: 1993

 

DOI:10.3109/10520299309105572

 

出版商: Taylor&Francis

 

关键词: Cartilage;Thionin;glycol methacrylate

 

数据来源: Taylor

 

摘要:

The usefulness of thionin for staining cartilage sections embedded in glycol meth-acrylate (GMA) and the effect of decalcification on cartilage sections embedded in paraffin and GMA were assessed. Short decalcification periods using 5% formic acid or 10% EDTA did not influence the staining properties or the morphology of cartilage matrix and chondrocytes. The standard stain safranin O-fast green for differential staining of cartilage was used as control in these experiments. Prolonged exposure of safranin P stained sections to fast green resulted in disappearance of the safranin O stained matrix, thereby hampering the quantitative measurement of negatively charged glycosaminoglycans (GAG). Thionin stained evenly throughout all cartilage layers, independent of the staining times. In contrast to safranin 0, thionin did not show meta-chromasia in nondehydrated cartilage sections, which made it more suitable for assessing cartilage quality in GMA embedded cartilage. To evaluate the selectivity of thionin staining in cartilage, chondroitinase ABC and trypsin digestions were carried out. Thionin staining was prevented by these enzymes in the territorial matrix, representing the interlacunar network and the chondrocyte capsule. Staining with thionin of the interterritorial matrix was only slightly reduced, possibly representing keratan sulfate and hyaluronic acid in cartilage of elderly patients. Comparison of thionin stained GMA embedded cartilage with safranin O stained paraffin embedded sections showed significant similarity in optical densitometry, indicative of the specificity of thionin bound to negatively charged GAG in cartilage. In GMA embedded cartilage morphology was relatively intact compared to paraffin embedded sections due to less shrinkage of chondrocytes and the interlacunar network.

 

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