Immunological Detection of Cytoskeletal Proteins In the Exoerythrocytic Stages of Malaria By Fluorescence and Confocal Laser Scanning Microscopy
作者:
ANDREAS SUHRBIER,
ROBERT E. SINDEN,
ANNA COUCHMAN,
SUZANNE L. FLECK,
SANJEEV KUMAR,
DUNCAN MCMILLAN,
期刊:
Journal of Eukaryotic Microbiology
(WILEY Available online 1993)
卷期:
Volume 40,
issue 1
页码: 18-23
ISSN:1066-5234
年代: 1993
DOI:10.1111/j.1550-7408.1993.tb04876.x
出版商: Blackwell Publishing Ltd
关键词: Cytoskeleton;indirect fluorescent antibody technique;Plasmodium
数据来源: WILEY
摘要:
ABSTRACT.Using monospecific antibodies, the presence and distribution of tubulin, actin, myosin, intermediate filaments, and lamins were examined in the exoerythrocytic liver schizont ofPlasmodium bergheiby conventional indirect fluorescent antibody methods and confocal laser scanning microscopy. the binding reactivity of the antibodies to parasite proteins was determined by Western blot analysis. the localisation of all antibodies in control host hepatocytes followed expected distributions in both uninfected and infected hepatocytes; by contrast, reactivity to the exoerythrocytic schizont was variable. the parasite reacted positively with selected anti‐tubulin, ‐actin, and ‐myosin antibodies in both fluorescence and Western blot analysis. Anti‐lamin antibodies were positive by confocal indirect fluorescent antibody labelling, but no labelling was detected with anti‐intermediate filament antibody. Within the technical limits of resolution of the methods as applied to asynchronous parasite infections, not one of the antibodies reacting positively with the parasite by the indirect fluorescent antibody technique could be shown to identify unequivocally the classic architectural features associated with their respective target organelles, i.e. microtubules, stress‐fibres or the nucle
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