AbstractA brief review of sugar stereochemistry is given. The separation of 84 different pairs of anomers was accomplished on both α‐ and β‐cyclodextrin columns. Five different mobile phases were evaluated. The separation of anomers temperature, flow rate, and so on. The separation of anomers that mutarotate is somewhat more difficult than those that do not. Prior knowledge as to the rate of mutaroation is useful so that the chromatographic conditions can be arranged to minimize any deleterious effects on the separ