SURFACE CHARACTERISTICS OF GROUP A STREPTOCOCCI WITH AND WITHOUT M‐PROTEIN
作者:
H. MIöRNER,
J. HAVLÍČEK,
G. KRONVALL,
期刊:
Acta Pathologica Microbiologica Scandinavica Series B: Microbiology
(WILEY Available online 1984)
卷期:
Volume 92B,
issue 1‐6
页码: 23-30
ISSN:0108-0180
年代: 1984
DOI:10.1111/j.1699-0463.1984.tb02789.x
出版商: Blackwell Publishing Ltd
关键词: Group A streptococci;surface characteristics;M‐protein
数据来源: WILEY
摘要:
Twenty M protein‐positive and eight M protein‐negative strains of group A streptococci were investigated with respect to surface hydrophobicity and amount of lipoteichoic acid (LTA). Surface hydrophobicity as determined in polymer two‐phase systems varied substantially between individual strains and there was no correlation to the presence of antiphagocytic M protein. The amount of LTA on the surface of the bacteria varied with hydrophobic affinity of the cells. Strains with a high content of surface LTA were found among both M‐positive and M‐negative streptococci. Cellular and extracellular LTA was estimated on six strains by the ability of hot phenol extracts and culture fluids to sensitize erythrocytes and by rocket immunoelectrophoretic quantitation. Differences in content of surface LTA did not correlate to differences in the total amount of cellular LTA. Pepsin digestion of M‐positive group A streptococci at suboptimal pH resulted in a loss of M antigen whereas surface LTA and the hydrophobic interaction liability was retained. The results indicate that the degree of surface hydrophobicity as measured by two‐phase partitioning is not correlated to either the type‐specific or the antiphagocytic moiety of M protein. The results support the correlation between surface LTA and surface hydrophobicity of group
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