Techniques for Clearing Ovules for Studies of Megagametophyte and Early Postfertilization Development inRhododendron
作者:
PalserBarbara F.,
RouseJohn L.,
WilliamsElizabeth G.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 4
页码: 207-218
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110068
出版商: Taylor&Francis
关键词: megagametophyte;ovule clearing;starch;tannin;hematoxylin;Rhododendron;Ericaceae
数据来源: Taylor
摘要:
Using ovule clearing, more than 33,600 ovules ofRhododendron nuttalliiT. W. Booth (Ericaceae) were examined for megagametophyte and early postfertilization stages at daily intervals from anthesis until 3 weeks after pollination. Pretreatments with amyloglucosidase to digest integumentary and gametophyte starch and Stockwell's bleach to remove tannins from the integumentary epidermis were necessary. Ovules were cleared by a combination or modifications of Heir's four-and-a-half or Stelly's hem-alum-methyl salicylate techniques and were observed using differential interference contrast optics. The method proved suitable for large-scale quantitative studies of ovule development and fertilization. A general protocol is suggested as a starting point for ovule clearing studies.
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