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Techniques for Clearing Ovules for Studies of Megagametophyte and Early Postfertilization Development inRhododendron

 

作者: PalserBarbara F.,   RouseJohn L.,   WilliamsElizabeth G.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1992)
卷期: Volume 67, issue 4  

页码: 207-218

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110068

 

出版商: Taylor&Francis

 

关键词: megagametophyte;ovule clearing;starch;tannin;hematoxylin;Rhododendron;Ericaceae

 

数据来源: Taylor

 

摘要:

Using ovule clearing, more than 33,600 ovules ofRhododendron nuttalliiT. W. Booth (Ericaceae) were examined for megagametophyte and early postfertilization stages at daily intervals from anthesis until 3 weeks after pollination. Pretreatments with amyloglucosidase to digest integumentary and gametophyte starch and Stockwell's bleach to remove tannins from the integumentary epidermis were necessary. Ovules were cleared by a combination or modifications of Heir's four-and-a-half or Stelly's hem-alum-methyl salicylate techniques and were observed using differential interference contrast optics. The method proved suitable for large-scale quantitative studies of ovule development and fertilization. A general protocol is suggested as a starting point for ovule clearing studies.

 

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