Two dimensional view of combination chemotherapy in vitro
作者:
Hans W. Grünwald,
Fred Rosner,
Yashar Hirshaut,
期刊:
American Journal of Hematology
(WILEY Available online 1978)
卷期:
Volume 4,
issue 1
页码: 35-46
ISSN:0361-8609
年代: 1978
DOI:10.1002/ajh.2830040106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: combination chemotherapy;lymphoid cell line;cytoxicity in vitro
数据来源: WILEY
摘要:
AbstractA newly developed micromethod to evaluate the cytotoxic effects of drugs on rapidly growing lymphoid cell lines is described, which permits evaluation of more than one variable. This technique allows a checkerboard titration of two drugs to determine the optimum concentration of each needed for the augmentation of the other's cell‐killing activity. Such studies have shown that the minimum concentration of vincristine capable of killing a human lymphoid cell population is 5.6 ng/ml. The addition of prednisolone at a concentration of 460 ng/ml reduces the minimum lethal concentration of vincristine to 1.6 ng/ml. When prednisolone is added to vincristine at concentrations of 2.8 μ/ml (a level readily achieved in the serum in vivo) concentrations of vincristine as low as 200 pg/ml are clearly cytotoxic. Similar potentiation of cytotoxicity following the addition of prednisolone has been demonstrated for cytosine arabinoside, but not for daunorubicin and only slightly for methotrexate. The micromethod for the study of optimal doses to be used in drug combinations is simple and gives highly reproducible results. It should be of considerable utility to anyone interested in screening a wide variety of drug concentrations for augmentation or synergistic effec
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