Histochemical demonstration of acidic glycosaminoglycans in the cell nuclei of the iris and other tissues
作者:
K. Sames,
期刊:
Cells Tissues Organs
(Karger Available online 1979)
卷期:
Volume 103,
issue 1
页码: 74-82
ISSN:1422-6405
年代: 1979
DOI:10.1159/000144997
出版商: S. Karger AG
关键词: Histochemistry;Glycosaminoglycans;Cell nucleus;Iris;DNA;Aging
数据来源: Karger
摘要:
Using histochemical methods, the presence of acidic glycosaminoglycans in the cell nuclei of 51 human irides and a series of monkey organs was demonstrated. In general, these substances are sensitive to testicular hyaluronidase and chondroitinase ABC and also to Streptomyces hyaluronidase, when using special staining methods. The specificity of testicular hyaluronidase was tested by. inhibition with heparin. By simultaneously staining with alcian blue and Feulgen, acidic glycosaminoglycans can be distinguished from the nucleic acids. Sporadically, hyaluronidase-resistant substances with a specific acidic glycosaminoglycan stainability occur. We assume the existence of various acidic glycosaminoglycans in the cell nuclei. Aging changes were not traceable with constancy.
点击下载:
PDF
(1288KB)
返 回