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Improved Immunoperoxidase Stain on Frozen Sections: An Avidin-Biotin-Peroxidase Complex (ABC) Technique

 

作者: MartyJoe,   KjeldsbergCarl R.,   GrooSteve,  

 

期刊: Journal of Histotechnology  (Taylor Available online 1982)
卷期: Volume 5, issue 2  

页码: 61-63

 

ISSN:0147-8885

 

年代: 1982

 

DOI:10.1179/his.1982.5.2.61

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

AbstractA modified Avidin-Biotin-Peroxidase complex (ABC) technique is described for use on frozen tissue sections and compared with the PAP technique. Increasing the incubation temperature and decreasing the incubation time resulted in (1) increased sensitivity with less background staining, (2) reduced turnaround time and (3) decreased cost with the modified ABC technique.

 

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