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Chromosome behaviour upon fertilization in eggs of the giant freshwater prawn,Macrobrachium rosenbergii(de Man)

 

作者: PRANEET DAMRONGPHOL,   NITTAYA EANGCHUAN,   BOONSERM POOLSANGUAN,  

 

期刊: Invertebrate Reproduction & Development  (Taylor Available online 1991)
卷期: Volume 19, issue 1  

页码: 45-49

 

ISSN:0792-4259

 

年代: 1991

 

DOI:10.1080/07924259.1991.9672155

 

出版商: Taylor & Francis Group

 

关键词: fertilization;meiotic maturation;chromosome manipulation;pronucleus;Macrobrachium rosenbergii;freshwater prawn

 

数据来源: Taylor

 

摘要:

Chronological changes of the chromosomes upon feritilization of the giant freshwater prawn,Macrobrachium rosenbergii(de Man), were followed. Egg chromosomes, within 2 min after spawning, were at the second meiotic metaphase whereas sperm nucleus retained a cupped shape of the base region. The egg chromosomes progressed to the second meiotic anaphase by 10–15 min and completed maturation by 20 min after spawning. Both female and male pronuclei were clearly differentiable at 1 hr after spawning. Female pronucleus was larger than the male pronucleus. But both pronuclei enlarged to an equal size when they were in proximity by 3 hr after spawning. Pronuclear fusion took place by 4 hr after spawning. The fused nucleus advanced into the first mitotic division rapidly so that the first mitotic metaphase or anaphase was observed by 5 hr after spawning. The first and the second nuclear divisions were completed by 6 and 8 hr after spawning, respectively. While the first and the second cytokineses occurred simultaneously at 9 hr after spawning resulting in a 4-cell embryo. Observations of chronology of the chromosomal events upon fertilization provide informations concerning the crucial times for further chromosome number manipulation.

 

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