The influence ofFagus sylvaticaL.,Quercus roburL.,Carpinus betulusL.,Tilia cordata Mill.andAcer platanoidesL. on pH(KCl) and organic matter content in the topsoil (0 to 5 cm), was studied at 70 mixed deciduous forest sites with different soil properties in the province of Scania, south Sweden. Samples at 50, 150 and 250 cm from the trunk were collected under canopies of individuals of different tree species growing side by side on initially similar soil. Analysis of differences included pairwise comparisons on each site. Compared toQ. robur,pH was 0.26 (at 50 cm) and 0.13 (at 150 and 250 cm) units lower forF. sylvatica. Acer platanoideshad 0.15 andT. cordata0.39 units higher pH thanQ. robur,as means of all distances. All differences were highly significant.Carpinus betulushad pH values very similar toQ. robur.The differences in soil organic matter content between the tree species were small and not significant.