Many plants and their products are used as sources of pharmaceuticals and as ingredients of traditional medicines, and are of value in new drug discovery. In this study, we fractionated the whole black seed proteins into twenty fractions by using Rotofor. The pH range was recorded as (2.63 to 10.46). Protein concentration range and percent protein recovered were 0.305 to 1.01 mg/mL and 5 to 17% respectively. A single 4 hr Rotofor run at 12W in the pH 3 to 10 range yielded well separated proteins into twenty fractions. Black seed (N. sativa) proteins isolated range between 200 kDa and 14 kDa when these fractions were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis.