An “on‐line” colorimetric method for repeated, rapid determinations of capillary diffusion capacity
作者:
Bengt Rippe,
Lars Stage,
期刊:
Acta Physiologica Scandinavica
(WILEY Available online 1978)
卷期:
Volume 102,
issue 1
页码: 108-115
ISSN:0001-6772
年代: 1978
DOI:10.1111/j.1748-1716.1978.tb06050.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
AbstractThe single injection indicator diffusion method for estimation of capillary diffusion capacity (Crone 1963) has been developed to provide directly visualized, continuous colorimetric recordings of the venous time‐concentration curves by means of a two‐colour densitometer system. Cardio‐Green, bound to albumin, is used as the non‐permeant (“reference”) tracer and Cr‐EDTA as the permeant one. The artificially perfused hindquarter muscle vascular bed of rats is used. Highly reproducible curve recordings can be obtained every fourth minute. Accidental disturbances of the recordings are readily detected. Thus, compared to the fractional venous sampling technique, the present technique has the great advantage of allowing immediately controlled and frequently repeated determinations of capillary diffusion capacity. It is therefore easily used together with measurements of filtration‐absorption events,e.g.for frequent quantitative comparisons of capillary diffusion and filtration capacities over a wide range of induced changes in perfused capillary surface area and/or capillary permeability. The main limitation is that the employed permeant tracer necessitates the use of erythrocyte
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